Western Blot Protocol

The following is AxioMx’s recommended western blot protocol. This protocol is applicable when primary antibodies are either FLAG®-labeled scFvs or IgGs. A. Buffers Lysis buffers Buffers may be stored at 4°C for several weeks or for up to a year aliquoted and stored at -20°C. Nonidet-P40 (NP40) buffer 150 mM NaCl 1.0% NP-40 (possible to…

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Titration Direct ELISA Protocol

The following is AxioMx’s recommended ELISA protocol. This protocol is applicable when primary antibodies are either FLAG®-labeled scFvs or IgGs. A. Buffers Antigen or antibody should be diluted in coating buffer to immobilize them to the wells: 10X PBS P80 g NaCl 2 g KCl 14.4 g NaHPO4 2.4 g KHPO4 Bring volume to 1L…

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